Device
Part:BBa_K533002
Designed by: ZHANG Yunxiao Group: iGEM11_Tsinghua (2011-09-29)
OmpA-HIV protease site-SH3
Usage and Biology
T7 promoter driven OmpA-HIV protease site-SH3 fusion protein expression cassette.
This part is used for binding to target protein with multi-proline sequence. The target protein can then be efficiently released by HIV protease.
Color Channel | E. coli with IPTG induction | E. coli without induction |
---|---|---|
mCherry | ||
DAPI | ||
merge |
The remaining were mixed with E. coli expressing OmpA-HIV protease at room temperature for 30 minutes to release the cargo.
After washing with distilled water twice, bacteria were fixed on a slip and stained with DAPI.
Color Channel | + E. coli expressing protease | + E. coli without protease |
---|---|---|
mCherry | ||
DAPI | ||
merge |
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 857
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//cds/membrane
//plasmid/expression/t7
//plasmid/expression/t7
negative_regulators | temperature, expressed below 20 centigrade |
positive_regulators | IPTG, 0.5mM |